The Goulburn River is subject to significant flow regulation and aseasonal flows resulting in implications for the condition of riverbank morphology and vegetation richness and abundance. To understand sediment and seed dynamics this 12 month study, as part of the Long Term Intervention Monitoring (LTIM) project, used artificial turf mats to collect sediments and seeds. Seedlings were grown and identified and sediments were analysed for load and calibre. This allowed assessment of relationships between sediments and seeds and characteristics such as flow events, season, contribution from tributaries, and the role of in-channel features such as bars and benches. The results are surprising, such as 2670 seedlings per square metre. This study has implications for flow operations, river ecology, and the physical integrity of regulated rivers.